Coding

Part:BBa_K5117026:Design

Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann   Group: iGEM24_TU-Dresden   (2024-09-28)


BhBglA-L1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 564
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

removal of restriction sites which are used for RFC[10] or Type IIS standards,

exchange of start codon (GTG > ATG) for usage in Bacillus subtilis, removal of the stop codon,

addition of a flexible linker encoding the amino acids GA

Source

Bacillus halodurans